anti-Tau, 3-repeat isoform RD3 antibody product blog
Tags: Antibody; Monoclonal Antibody; Tau, 3-repeat isoform RD3; anti-Tau, 3-repeat isoform RD3 antibody;
The Tau, 3-repeat isoform RD3 n/a (Catalog #MBS600324) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Tau, 3-repeat isoform RD3 (Tau Protein, Microtubule-associated Protein, MAP) (Azide, BSA & Glycerol Free) reacts with Human, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Tau, 3-repeat isoform RD3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in Western Blot, Immunohistochemistry and Differential Detection of Tauopathies.
Dilution: Western Blot: 1:1000-1:4000 detects Tau (3-repeat isoform RD3) in lysates from rat brain cytosol fraction. Rat brain cytosol fraction was resolved by electrophoresis, transferred to nitrocellulose and probed with T1030-40B1. Proteins were visualized using an IgG (HRP) Gt x Mo and a chemiluminescence detection system.
Immunohistochemistry: Detects Tau (3-repeat isoform RD3) in autoclaved paraffin brain sections (1).
Differential Detection of Tauopathies: See Ref. 2. Researchers should empirically determine the suitability of the Tau, 3-repeat isoform RD3 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Tau, 3-repeat isoform RD3 (Tau Protein, Microtubule-associated Protein, MAP) (Azide, BSA & Glycerol Free) with the following immunoassay(s):
Western Blot (WB) (WB: Rat brain cytosol fraction was probed with MBS600324(1:2000 dilution).)
Tau is a heterogeneous group of proteins that range in molecular weight from 50-67kD. They are instumental in the assembly of microtubules and have been associated with some disease states. In normal brain, tau is localized in the axons of the neurons but in some neuropathological lesions, it accumulates within the body of the neuron. Tau protein is a microtubule-associated protein predominantly localized to neuronal axons. Tau promotes tubulin polymerization and the stabilization of microtubules. Hyperphosphorylated forms of Tau are the major components of paired helical filaments (PHF) found in the neurofibrillary tangles which characterize Alzheimer\'s disease. Alternate splicing of tau mRNA, glycosylation, and differential phosphorylation contribute to the heterogeneity of tau.
Immunogen: Bovine thyroglobulin conjugated synthetic peptide corresponding to amino acids 209-224, KHQPGGGKVQIVYKPV of human Tau (3- repeat isoform RD3). Region is flanking junction coded by adjacent exons 9 & 11 in the absence of exon 10. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Tau, 3-repeat isoform RD3 are readily searchable from our website. Different antibodies against the same target such as Tau, 3-repeat isoform RD3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.