|The Tamm-Horsfall Glycoprotein n/a (Catalog #MBS615852) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Tamm-Horsfall Glycoprotein (Uromucoid, THgp, THP, Uromodulin, UMOD) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Tamm-Horsfall Glycoprotein can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunohistochemistry (IHC).
Suitable for use in Immunohistochemistry and ELISA.
Dilution: Immunohistochemistry (Frozen sections only):1:25- 1:100.
ELISA: 1:5000- 1:20,000. Researchers should empirically determine the suitability of the Tamm-Horsfall Glycoprotein n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Tamm-Horsfall Glycoprotein n/a product has the following accession number(s) (GI #2119691) (NCBI Accession #I84634). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Tamm-Horsfall glycoprotein (THP), which is synthesized by renal tubular cells, is the most abundant protein in normal human urine. Although its physiological function remains unclear, it has been proposed that THP may act as a defence factor against urinary tract infections by inhibiting the binding of S- and P-fimbriated Escherichia coli to renal epithelial cells. Because THP-related proteins are also found in the superficial layers of the oral mucosa, THP may interfere with the cytoadherence of pathogenic bacteria that colonize mucosal surfaces other than those of the urogenital tract. It has been shown that THP binds to virulent Bordetella pertussis and reduces its adherence to both renal and pulmonary epithelial cells. This cytoadherence inhibitory effect was not observed with a B. pertussis mutant lacking the pertussis toxin (PTX) operon, and was dependent on the direct interaction of THP with the S2 subunit within the PTX B oligomer. It has also been shown that the glycosylation moiety of THP is crucial for its binding to PTX. The THP-PTX interaction was exploited to develop an affinity chromatography method that allows an one-step purification of active PTX. These observations suggest that besides its anti-adherence activity, THP may also trap toxins produced by pathogenic bacteria that colonize mucosal surfaces.
Immunogen: Native purified human Tamm-Horsfall glycoprotein (uromucoid) from urine. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Tamm-Horsfall Glycoprotein are readily searchable from our website. Different antibodies against the same target such as Tamm-Horsfall Glycoprotein may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.