anti-STEAP1 antibody product blog
Tags: Antibody; Monoclonal Antibody; anti-STEAP1 antibody; STEAP1;
The STEAP1 steap1 (Catalog #MBS200111) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The STEAP1 antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s STEAP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC).The antibody has been tested by ELISA, Western blot, immunofluorescence and immunohistochemistry analysis to assure specificity and reactivity.
Recommended dilution range for Western blot is 1:1,000~1:2,000, immunofluorescence 1:100 ~1:300 and immunohistochemistry analysis is 1:50~100.
Recommended starting dilution for Western blot is 1:1,000, Immunofluorescence 1:100 and Immunohistochemistry analysis is 1:50. Researchers should empirically determine the suitability of the STEAP1 steap1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The STEAP1 steap1 product has the following accession number(s) (GI #9558759) (NCBI Accession #NP_036581) (Uniprot Accession #Q9UHE8). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the STEAP1 antibody with the following immunoassay(s):
Western Blot (WB) (Cell lysates of LNcap (30ug) and Rat testis (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human STEAP1 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Immunohistochemistry (IHC) (Paraffin embedded sections of human breast cancer and prostate hyperplasia tissue were incubated with anti-human STEAP1 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB))
Immunofluorescence (IF) (Immunofluorescence staining of human LNCap cell colony with monoclonal anti- human STEAP1 antibody (J2D2) )
Six-transmembrane epithelial antigen of the prostate (STEAP) is expressed predominantly in human prostate tissue and in other common malignancies including prostate, bladder, colon, and ovarian carcinomas, and in Ewing\'s sarcoma, suggesting that it could function as an almost universal tumor antigen. Immunohistochemical analysis indicated STEAP-1 localization at cell-cell junctions of the secretory epithelium of prostate and prostate cancer cells. The favorable expression profiling of STEAP-1 in normal and cancer tissues suggested its potential use as a target for immunotherapy.
Antigen Species: Human
Immunogen: Recombinant human STEAP1 (1-70aa) purified from E. coli. Clone Note: Anti-human STEAP1 mAb, clone J2D2, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human STEAP1 protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing STEAP1 are readily searchable from our website. Different antibodies against the same target such as STEAP1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.