Anti-ssDNA elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; Anti-SSDNA elisa kit; Anti-SSDNA; Anti-Single Stranded DNA;
The Mouse ssDNA n/a (Catalog #MBS724935) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS724935 ELISA Kit recognizes Mouse ssDNA.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse Anti-Single Stranded DNA ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: ssDNA-Ab ELISA kit applies the competitive enzyme immunoassay technique utilizing Single Stranded Deoxyribonucleic Acid antigen and an ssDNA-Ab-HRP conjugate. The assay sample and buffer are incubated together with ssDNA-Ab-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ssDNA-Ab concentration since ssDNA-Ab from samples and ssDNA-Ab-HRP conjugate compete for the Single Stranded Deoxyribonucleic Acid antigen binding site. Since the number of sites is limited, as more sites are occupied by ssDNA-Ab from the sample, fewer sites are left to bind ssDNA-Ab-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ssDNA-Ab concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Sandwich
Detection Range: 0-250 U/mL
Sensitivity: 1.0 U/mL. Intended Uses: This ssDNA-Ab ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ssDNA-Ab. This ELISA kit for research use only, not for therapeutic applications!. Mouse Anti-Single Stranded DNA ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.