anti-SPRED1 antibody product blog
Tags: Antibody; Polyclonal Antibody; Spred1; anti-SPRED1 antibody;
The SPRED1 spred1 (Catalog #MBS151493) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Spred1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Spred1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).Spred1 antibody can be used for detection of Spred1 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. Researchers should empirically determine the suitability of the SPRED1 spred1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SPRED1 spred1 product has the following accession number(s) (GI #22749221) (NCBI Accession #NP_689807) (Uniprot Accession #Q7Z699). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Spred1 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of Spred1 in mouse brain tissue lysate with Spred1 antibody at (A) 1 and (B) 2 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of Spred1 in human brain tissue with Spred1 antibody at 2.5 μg/mL.)
Spred1 Antibody: The Ras-MAP kinase pathway is essential for the differentiation of neuronal cells and myocytes; it is inhibited by Spred1, a member of the Sprouty family of proteins. Spred1 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS). This Spred1 antibody is predicted to have no cross-reactivity to Spred2 or Spred3.
Conjugate: Unconjugated
Immunogen: Spred1 antibody was raised against a 20 amino acid synthetic peptide near the center of the human Spred1.
Buffer: Spred1 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SPRED1 are readily searchable from our website. Different antibodies against the same target such as SPRED1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.