anti-SOX3 antibody product blog
Tags: Antibody; SOX3; Polyclonal Antibody; anti-SOX3 antibody;
The SOX3 sox3 (Catalog #MBS422374) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SOX3 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog and may cross-react with other species as described in the data sheet. MyBioSource\'s SOX3 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).Peptide ELISA: Antibody detection limit dilution 1: 8000.
Immunohistochemistry: In paraffin embedded Human Cerebrl Cortex shows nuclear staining at verious intensities along the neuronal cells. Recommended concentration, 3-5ug/ml.
Western Blot: Approx 42kDa band observed in Human Brain (Cerebellum, Cerebral Cortex and Frontal Cortex) lysates (calculated MW of 45.2kDa according to NP_005625.2). Recommended concentration: 0.5-1.5ug/ml. Researchers should empirically determine the suitability of the SOX3 sox3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SOX3 sox3 product has the following accession number(s) (GI #30061556) (NCBI Accession #NP_005625.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
DAASPLPGGR LHGVH.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-SOX3 Antibody with the following immunoassay(s):
Western Blot (WB) ((0.5ug/ml) staining of Human Cerebral Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC) ((3.8ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunogen: Peptide with sequence C-DAASPLPGGRLHGVH, from the C Terminus of the protein sequence according to NP_005625.2.
Epitope: C Terminus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SOX3 are readily searchable from our website. Different antibodies against the same target such as SOX3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Abnormalities, Multiple, Cell Transformation, Neoplastic, Disease Models, Animal, Immune System Diseases, Lung Diseases, Neoplasm Metastasis, Nervous System Diseases, Nervous System Malformations, Neurobehavioral Manifestations, Panhypopituitarism X-linked are some of the diseases may be linked to Goat anti-SOX3 Antibody. Brain, Embryonic Tissue, Testis tissues are correlated with this protein.