anti-SOD1 antibody product blog
Tags: Antibody; SOD1; Polyclonal Antibody; anti-SOD1 antibody; Superoxide Dismutase 1;
The SOD1 sod1 (Catalog #MBS175666) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Superoxide Dismutase 1 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Superoxide Dismutase 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC.WB: Concentration: 0.1-0.5ug/ml; Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1ug/ml; Tested Species: Human, Mouse, Rat; Antigen Retrieval: By Heat
IHC-F: Concentration: 0.5-1ug/ml; Tested Species: Human; Predicted Species: Mouse, Rat
ICC: 0.5-1.0ug/ml; Tested Species: Human
Tested Species: In-house tested species with positives results.
Predicted Species: Species predicted to be fit for the product based on sequence similarties.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the SOD1 sod1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SOD1 sod1 product has the following accession number(s) (GI #134611) (NCBI Accession #P00441.2) (Uniprot Accession #P00441). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Superoxide Dismutase 1 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-SOD1 antibody, MBS175666, Western blotting
Lane 1: COLO320 Cell Lysate
Lane 2: SMMC Cell Lysate
)
Immunohistochemistry (IHC) (Anti-SOD1 antibody, MBS175666, IHC(P)
IHC(P): Human Mammary Cancer Tissue )
Description: Rabbit IgG polyclonal antibody for Superoxide dismutase[Cu-Zn](SOD1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.
Background: Superoxide dismutases(SOD) are a class of enzymes that catalyze the dismutation of superoxide into oxygen and hydrogen peroxide. As such, they are an important antioxidant defense in nearly all cells exposed to oxygen. One of the exceedingly rare exceptions is Lactobacillus plantarum and related lactobacilli, which use a different mechanism.Cu,Zn-SOD was found widely distributed in the cell cytosol and in the cell nucleus, consistent with it being a soluble cytosolic protein. Mitochondria and secretory compartments did not label for this protein. In human cells, peroxisomes showed a labeling density slightly less than that of cytoplasm.
Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of Human Superoxide Dismutase 1 (113-129aa IIGRTLVVHEKADDLGK), different from the mouse sequence by two amino acids. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.