anti-SOCS3 antibody product blog
Tags: Antibody; Polyclonal Antibody; SOCS3; anti-SOCS3 antibody;
The SOCS3 socs3 (Catalog #MBS175265) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SOCS3 antibody reacts with Reactes with: HumanPredict to work with: Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s SOCS3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin.
Western Blot
Concentration: 0.1- 0.5 ug/ml
Tested Species: Human
Predicted Species: Mouse
Immunohistochemistry- (Paraffin- embedded Section)
Concentration: 0.5- 1 ug/ml
Tested Species: Human
Predicted Species: Mouse
Antigen Retrieval: By Heat
Tested Species: In house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0 for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the SOCS3 socs3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SOCS3 socs3 product has the following accession number(s) (GI #20178094) (NCBI Accession #O14543.1) (Uniprot Accession #O14543). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-SOCS3 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-SOCS3 antibody, MBS175265, Western blotting
Lane 1: JURKAT Cell Lysate
Lane 2: CEM Cell Lysate
Lane 3: RAJI Cell Lysate
)
Immunohistochemistry (IHC) (Anti-SOCS3 antibody, MBS175265, IHC(P)
IHC(P): Human Tonsil Tissue )
Description: Rabbit IgG polyclonal antibody for Suppressor of cytokine signaling 3(SOCS3) detection. Tested with WB, IHC-P in Human, Mouse.
Background: SOCS3(Suppressor of cytokine signaling 3) is a protein that in humans is encoded by the SOCS3 gene. SOCS3 is transiently expressed by multiple cell lineages within the immune system and functions predominantly as a negative regulator of cytokines that activate the JAK-STAT3 pathway. This gene encodes a member of the STAT-induced STAT inhibitor(SSI), also known as suppressor of cytokine signaling(SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene is induced by various cytokines, including IL6, IL10, and interferon(IFN)-gamma. The protein encoded by this gene can bind to JAK2 kinase, and inhibit the activity of JAK2 kinase. For signaling of IL-6, Epo, GCSF and Leptin, binding of SOCS3 to the respective cytokine receptor has been found to be crucial for the inhibitory function of SOCS3. Studies of the mouse counterpart of this gene suggested the roles of this gene in the negative regulation of fetal liver hematopoiesis, and placental development.