anti-SNAP25 antibody product blog
Tags: Antibody; SNAP25; Monoclonal Antibody; anti-SNAP25 antibody;
The SNAP25 snap25 (Catalog #MBS200064) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The SNAP25 antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s SNAP25 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB) (Cell lysate). Researchers should empirically determine the suitability of the SNAP25 snap25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The SNAP25 snap25 product has the following accession number(s) (GI #18765735) (NCBI Accession #NP_570824) (Uniprot Accession #P60880). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SNAP25 antibody with the following immunoassay(s):
Western Blot (WB) (The extracts of mouse brain (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human SNAP25 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human SNAP25 antibody (1:3000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: 293T cell lysate Lane 2.: SNAP25 Transfected 293T cell lysate)
Immunofluorescence (IF) (ICC/IF analysis of SNAP25 in U87MG cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human SNAP25 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
The synaptosomal-associated protein (SNAP25) is an essential component of the core complex that mediates presynaptic vesicle trafficking. Thus, SNAP25 is directly involved in the release of neurotransmitters.
Antigen Species: Human
Immunogen: Recombinant human SNAP25 (1-206aa) purified from E. coli. Clone Note: Anti-human SNAP25 mAb, clone 4E11, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human SNAP25 protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SNAP25 are readily searchable from our website. Different antibodies against the same target such as SNAP25 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Brain Diseases, Cognition Disorders, Memory Disorders, Movement Disorders, Nervous System Diseases, Neurobehavioral Manifestations, Pain, Paralysis, Poisoning, Seizures are some of the diseases may be linked to SNAP25 antibody.