anti-SMAD6 antibody product blog
Tags: Antibody; Polyclonal Antibody; SMAD6; anti-SMAD6 antibody;
The SMAD6 smad6 (Catalog #MBS241862) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal (IgG) to Human SMAD6 reacts with Human, PigPredicted Reactivity: Bovine, Dog (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. MyBioSource\'s SMAD6 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB).
IHC-P (5 ug/ml), WB (0.5 - 2 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the SMAD6 smad6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SMAD6 smad6 product has the following accession number(s) (GI #92859871) (NCBI Accession #NP_005576.3) (Uniprot Accession #O43541). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Polyclonal (IgG) to Human SMAD6 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-SMAD6 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Target Species: Human
Immunogen Description: This antibody was developed against synthetic peptides corresponding to amino acids 85-99 (RAGGPPRPMSEPGAG)and amino acids 372-388 (NLEQRSESVRRTRSKIG)of human SMAD6 (GenBank Accession No. AAH12986.1). Immunogen Type: Synthetic peptide
Immunogen: SMAD6 antibody was raised against this antibody was developed against synthetic peptides corresponding to amino acids 85-99 (RAGGPPRPMSEPGAG)and amino acids 372-388 (NLEQRSESVRRTRSKIG)of human SMAD6 (GenBank Accession No. AAH12986.1). These peptide sequences are specific to SMAD6, and have high homology to SMAD6in multiple species. Percent identity by BLAST analysis: Human, Pig (100%); Dog, Bovine, Opossum (93%); Gibbon, Marmoset, Rat (87%); Elephant (80%).
Antigen Modification: aa85-99. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SMAD6 are readily searchable from our website. Different antibodies against the same target such as SMAD6 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. SMAD6 also interacts with the following gene(s): BMPR2, HOXC8, SMAD1, SMAD4, SMAD5, SMAD7, SMURF1, SMURF2, TAB1.