anti-SMAD4 antibody product blog
Tags: Antibody; Monoclonal Antibody; SMAD4; anti-SMAD4 antibody;
The SMAD4 smad4 (Catalog #MBS603759) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Smad4 (DPC4, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma) (BSA & Azide Free) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Smad4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF).Suitable for use in Immunofluoresence, Immunoprecipitation, and Western Blot.,, Recommended Dilutions:, Immunoprecipitation (Native and denatured): 2ug/mg protein lysate. Use Protein G., Western Blot: 1-2ug/ml for 2hrs at RT, Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the SMAD4 smad4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SMAD4 smad4 product has the following accession number(s) (GI #13603414) (Uniprot Accession #Q9BYG6). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Smad4 (DPC4, Small Mothers Against Decapentaplegic Deleted in Pancreatic Carcinoma) (BSA & Azide Free) with the following immunoassay(s):
Testing Data
Testing Data
DPC4 (also named SMAD4; small mothers against decapentaplegic deleted in pancreatic carcinoma) belongs to the family of SMAD molecules comprising essential mediators of transforming growth factor beta (TGF-b) signalling. To date, seven members of this family have been identified, each of which plays a specific and separate role in mediating TGF-beta superfamily gene transcription. At least two different members, Smad2 and Smad4/DPC4, have been implicated in human cancer and appear to have tumor-suppressor functions whose inactivation may play an important role in the histogenesis of pancreatic and colorectal malignancy and possibly other human cancers as well.
Immunogen: Purified recombinant DPC4 protein. Molecular Weight: 60kD. Cellular Localization: Nuclear. Positive Control: LS174T, HT29, Raji and Jurkat cells. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SMAD4 are readily searchable from our website. Different antibodies against the same target such as SMAD4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Embryonic Tissue, Kidney, Liver, Lung, Muscle, Pancreas, Skin, Vascular tissues are correlated with this protein.