anti-SMAD4 antibody product blog
Tags: Antibody; Monoclonal Antibody; SMAD4; anti-SMAD4 antibody; Smad4(DPC4);
The SMAD4 smad4 (Catalog #MBS175074) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Smad4(DPC4) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC).WB: 2-4ug/ml with the appropriate system to detect Smad4 in cells and tissues.
Other applications have not been tested. Optimal dilutions should be determined by end user. Researchers should empirically determine the suitability of the SMAD4 smad4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SMAD4 smad4 product has the following accession number(s) (GI #13959561) (NCBI Accession #Q13485.1) (Uniprot Accession #Q13485). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Smad4(DPC4) antibody (monoclonal) with the following immunoassay(s):
Western Blot (WB) (Anti-Smad4(DPC4) antibody (monoclonal), MBS175074, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
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Description: Mouse IgG monoclonal antibody for Smad4 (DPC4), SMAD family member 4 (SMAD4) detection. Tested with WB, ICC in Human. No cross reactivity with other proteins. Uniprot ID: Q13485
Background: SMAD4 plays a pivotal role in signal transduction of the transforming growth factor beta superfamily cytokines by mediating transcriptional activation of target genes. Smad4 signalling in T cells is required for suppression of gastrointestinal cancer. Mutational inactivation of SMAD4 causes TGF-beta unresponsiveness and gave a basis for understanding the physiologic role of this gene in tumorigenesis. Mutations in DPC4(SMAD4) cause juvenile polyposis syndrome, but only account for a minority of cases.
Immunogen: Recombinant human Smad4 (DPC4). Contents: Mouse�ascites�fluid,�1.2%�sodium�acetate,�2mg�BSA,�with�0.01mg�NaN3�as�preservative.
Reconstitution: 1.2% sodium acetate or neutral PBS. If 1ml of PBS is used, the antibody concentration will be 100ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SMAD4 are readily searchable from our website. Different antibodies against the same target such as SMAD4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. SMAD4 also interacts with the following gene(s): EP300, FOXH1, PIAS4, SKI, SMAD1, SMAD2, SMAD3, SP1, TRIM33. Blood, Bone, Embryonic Tissue, Kidney, Liver, Lung, Muscle, Pancreas, Skin, Vascular tissues are correlated with this protein.