anti-Slc11a1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-Slc11a1 antibody; Slc11a1; Natural Resistance Associated Macrophage Protein with IRE;
The Slc11a1 slc11a1 (Catalog #MBS617851) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Natural Resistance Associated Macrophage Protein with IRE (Nramp2 with IRE) (formerly M1252-06) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Natural Resistance Associated Macrophage Protein with IRE can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1-10ug/ml (ECL).
ELISA: 0.5-1ug/ml. Coat ELISA plates at 1ug/ml of Control Peptide. Researchers should empirically determine the suitability of the Slc11a1 slc11a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Slc11a1 slc11a1 product has the following accession number(s) (GI #505156) (Uniprot Accession #P41251). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Iron is an essential element for a variety physiological process. A variety of proteins are involved in the transport of iron. Most dietary iron exists in the form of ferric iron complexes which must be reduced to yield ferrous ion before it can be taken up by protein transporters such as DCT1 (divalent cation transporter)/NRAMP2/DMT1. These transporters are especially active in small intestine where most dietary iron absorption is conducted. Ferrous Fe (II) is very unstable at physiological pH and quickly oxidized to ferric Fe (III). Highly specialized transmembrane electron transport system, maintained by ferric reductases, is required for the availability of intracellular ferrous ion. A second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568aa, ~65% identity with NRAMP1). NRAMP2 expression is more ubiquitous and has been detected in most tissues. NRAMP2 expression is more pronounced in brain, thymus, proximal intestine, kidney and bone marrow. It is dramatically up regulated by iron starvation in the intestine. NRAMP2 gene produces two alternatively spliced transcripts generated by alternative use of two 3\' exons encoding distinct C-termini of the protein as well as distinct 3\' untranslated regions (UTRs). One Nramp2 mRNA contains an iron-responsive element (IRE) in its 3\'UTR. The IRE is an RNA secondary structure present in the 5\'- or the 3\'-UTR of animal mRNAs encoding proteins involved in iron metabolism. The second Nramp2 splice isoform (without-IRE, isoform II) encodes a protein in which the C-terminal 18aa of the IRE form (with IRE, isoform I) is replaced by a novel 25aa segment and codes for a distinct 3\' UTR lacking the IRE. The two isoforms are differentially localized and regulated in GI tract and kidney. It has been demonstrated that the Nramp2 gene is mutated (Gly185 to Arg at TM4) in both the mk and Belgrade (b) animal models exhibiting a severe microcytic hypochromic anemia marked by a defect in iron absorption by intestinal cells and in erythroid iron use.