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anti-SIRT2 antibody product blog

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Posted on 2013-12-22 10:47:25 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; SIRT2; anti-SIRT2 antibody;
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The SIRT2 sirt2 (Catalog #MBS150756) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SIRT2 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s SIRT2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
SIRT2 antibody can be used for detection of SIRT2 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 5 mug/mL. Researchers should empirically determine the suitability of the SIRT2 sirt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The SIRT2 sirt2 product has the following accession number(s) (GI #13775600) (NCBI Accession #NP_036369) (Uniprot Accession #Q8IXJ6). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SIRT2 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of SIRT2 in Mouse brain lysate with SIRT2 antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB) SIRT2.

Immunohistochemistry (IHC) (Immunohistochemical staining of Human Brain tissue using SIRT2 antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC) SIRT2.

Immunofluorescence (IF) (Immunofluorescence of SIRT2 in Human Brain tissue with SIRT2 antibody at 5 μg/mL.)
Immunofluorescence (IF) SIRT2.

SIRT2 Antibody: The founding member of the sirtuin protein family was the silent information regulator 2 protein (Sir2p) of Saccharomyces cervisiae, an NAD+-dependent histone deacetylase (HDAC) that regulates chromatin silencing. The SIR2 family of genes are highly conserved from prokaryotes to eukaryotes. Mammals have seven homologs of Sir2p, SIRT1-7, which are involved in diverse processes ranging from transcriptional regulation, cell cycle progression and DNA-damage repair to aging. SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules and can deacetylate alpha-tubulin and regulate progression through the cell cycle. Most Sirtuins are highly expressed in brain and testis, while Sirt2 expression is higher in fetal relative to adult brain. Recent studies on SIRT2 support the therapeutic utility of inhibitors for the treatment of neurodegenerative diseases such as Parkinson\'s disease.

Conjugate: Unconjugated
Immunogen: SIRT2 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of the human SIRT2.
Buffer: SIRT2 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SIRT2 are readily searchable from our website. Different antibodies against the same target such as SIRT2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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