anti-SH2D1A antibody product blog
Tags: Antibody; Polyclonal Antibody; SH2D1A; SH2D1A/SAP; anti-SH2D1A antibody;
The SH2D1A sh2d1a (Catalog #MBS420671) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SH2D1A/SAP Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s SH2D1A/SAP can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunoprecipitation (IP).Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 16kDa band observed in Human Thymus lysates (calculated MW of 14.2xkDa according to NP_002342.1). In transfected HEK293 transiently expressing SH2D1A a band of approx.16kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.3-1ug/ml.
Immunoprecipitation: This antibody was deemed fit for IP under native conditions (observations from anonymous customer). Researchers should empirically determine the suitability of the SH2D1A sh2d1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SH2D1A sh2d1a product has the following accession number(s) (GI #4506923) (NCBI Accession #NP_002342.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
HKRYFRKIKN.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-SH2D1A/SAP Antibody with the following immunoassay(s):
Western Blot (WB) (Staining (0.3ug/ml) of Human Thymus lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB) (HEK293 overexpressing SH2D1A (RC204723) and probed (mock transfection in first lane), tested by Origene.)
Immunogen: Peptide with sequence C-HKRYFRKIKN, from the internal region of the protein sequence according to NP_002342.1; NP_001108409.1.
Epitope: Internal region. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SH2D1A are readily searchable from our website. Different antibodies against the same target such as SH2D1A may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone Marrow, Liver, Lung, Pharynx, Prostate, Spleen, Testis, Thymus tissues are correlated with this protein.