anti-SERPINA1 antibody product blog
Tags: Antibody; Polyclonal Antibody; SERPINA1; anti-SERPINA1 antibody; alpha 1 Trypsin;
The SERPINA1 serpina1 (Catalog #MBS534237) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The alpha 1 Trypsin antibody reacts with human alpha-1-trypsin and may cross-react with other species as described in the data sheet. MyBioSource\'s alpha 1 Trypsin can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).ELISA: 1:20,000-1:100,000
WB: 1:2,000-1:10,000. Researchers should empirically determine the suitability of the SERPINA1 serpina1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SERPINA1 serpina1 product has the following accession number(s) (GI #177831) (NCBI Accession #AAB59495.1) (Uniprot Accession #P01009). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the alpha 1 Trypsin antibody with the following immunoassay(s):
Western Blot (WB) (Western Blot of Alpha 1 anti trypsin antibody. Lane 1: molecular weight. Lane 2: Human transferrin (green); Alpha 1 anti trypsin (red); and Human IgG (Blue) in a multiplex fluorescent western blot of human serum. Load: 35 ug per lane. Each primary antibody was diluted to 1:1000 for overnight at 4 deg C. Rabbit IgG (H&L) antibody DyLight649 Conjugated (green), Goat IgG (H&L) antibody DyLight549 Conjugated (red), Mouse IgG (H&L) antibody DyLight488 Conjugated (blue)secondary antibody at 1:10,000 for ~ 1hr at 4 degrees. Block: IRdye blocking buffer overnight at 4 deg C)
Western Blot (WB) (Western Blot of Trypsin antibody-Multiplex Fluorescent Western blot. Lane 1: molecular weight. Lane 2: alpha 1 Antitrypsin (red), Transferrin, and Mouse anti GST were used in a multiplex system to detect target proteins under reducing conditions in albumin depleted human serum with 320 ng of added GST. Load: 35 ug per lane. Each primary antibody at 1:1000 for overnight at 4 deg C. DyLight549 Donkey anti-Rabbit IgG (green), DyLight 488 Donkey anti-Mouse IgG (blue), and DyLight 649 Donkey anti-Goat IgG (red) secondary antibody at 1:10,000 for 30 min at RT. Block: Blocking buffer at 30 min RT)
Western Blot (WB) (Western Blot of alpha 1 Antitrypsin antibody. Lane 1: alpha 1 Antitrypsin under reducing conditions. Lane 2: none. Load: 1 ug per lane. alpha 1 Antitrypsin antibody at 1:3000 for overnight at 4 deg C. Dylight 488 conjugated Donkey anti Goat secondary antibody at 1:10,000 for 45 min at RT. Block: Blocking buffer overnight at 4 deg C. Size: 55kDa for a-1 Anti-Trypsin.)
Goat polyclonal alpha 1 Trypsin antibody.
Biological Significance: Alpha 1-Antitrypsin or alpha1-antitrypsin (A1AT) is a protease inhibitor belonging to the serpin superfamily. It is generally known as serum trypsin inhibitor. Alpha 1-antitrypsin is also referred to as alpha-1 proteinase inhibitor (A1PI) because it inhibits a wide variety of proteases.