anti-SDHD antibody product blog
Tags: Antibody; Polyclonal Antibody; SDHD; anti-SDHD antibody;
The SDHD sdhd (Catalog #MBS150810) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SDHD Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s SDHD can be used in a range of immunoassay formats including, but not limited to, Antibody validated: Western Blot in mouse samples: Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All others applications and species not yet tested.SDHD antibody can be used for detection of SDHD by Western blot at 1 - 2 ug/mL.
Antibody can also be used for immunocytochemistry starting at 2.5 ug/mL.
For immunofluorescence start at 2.5 ug/mL.
Optimal dilutions for each application to be determined by the researcher. Researchers should empirically determine the suitability of the SDHD sdhd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SDHD sdhd product has the following accession number(s) (GI #4506865) (NCBI Accession #NP_002993) (Uniprot Accession #O14521). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the SDHD Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of SDHD in EL4 cell lysate with SDHD antibody at (A) 1 and (B) 2 μg/mL.)
Immunocytochemistry (IHC) (Immunocytochemistry of SDHD in EL4 cells with SDHD antibody at 2.5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of SDHD in EL4 cells with SDHD antibody at 20 μg/mL.)
SDHD Antibody: The mitochondrial succinate dehydrogenase complex subunit D (SDHD) is one of four proteins that make up the tricarboxylic cycle enzyme succinate dehydrogenase (SCH). Studies have shown that mutations in SDHD often leads to hereditary paragangliomas, usually benign tumors of the autonomic nervous system, suggesting that SDHD also plays a role as a tumor-suppressor gene. In one family with a nonsense mutation (R22X) in the SDHD gene, a loss of heterozygosity was found in the paragangliomas, and within these tumors the enzymatic activity of Complex II in the mitochondrial respiratory chain was completely abolished. Furthermore, high levels of angiogenic factors EPAS1 and VEGF was observed, which may stimulate tumor growth.
Conjugate: Unconjugated
Immunogen: The immunogen is located within amino acids 30 - 80 of SDHD. Homology: Predicted species reactividy based on immunogen sequence: Bovine: (100%), Sheep (100%), Pig (80%)
SDHD antibody was raised against a 15 amino acid synthetic peptide near the center of human SDHD. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SDHD are readily searchable from our website. Different antibodies against the same target such as SDHD may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.