anti-SCARF1 antibody product blog
Tags: Antibody; Polyclonal Antibody; SCARF1/SREC; anti-SCARF1 antibody; SCARF1;
The SCARF1 scarf1 (Catalog #MBS420351) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-SCARF1/SREC Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. MyBioSource\'s SCARF1/SREC can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 85-90kDa band observed in Human Heart lysates (calculated MW of 87.4kDa according to NP_003684;). Recommended concentration: 0.3-1. 0ug/ml. An additional faint band of 28kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
Immunocytochemsitry/Immunofluorescence: This antibody has been successfully used in ICC/IF for HUVEC cells. Researchers should empirically determine the suitability of the SCARF1 scarf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The SCARF1 scarf1 product has the following accession number(s) (GI #33598929) (NCBI Accession #NP_003684.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
SELDPKGQHV C.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-SCARF1/SREC Antibody with the following immunoassay(s):
Western Blot (WB) ((0.3ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunising peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunogen: Peptide with sequence SELDPKGQHVC, from the internal region of the protein sequence according to NP_003684.2; NP_663325.1; NP_663327.2.
Epitope: Internal region. Note: This antibody is expected to recognise all three reported isoforms (NP_003684.2; NP_663325.1; NP_663327.2). Epitope corresponding to aa 20-31, mapping to the mature human SCARF1 / SREC1 protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing SCARF1 are readily searchable from our website. Different antibodies against the same target such as SCARF1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Atherosclerosis, Eye Abnormalities, Fatty Liver, Genital Diseases, Female, Inflammation, Kidney Diseases, Liver Neoplasms, Male Urogenital Diseases, Musculoskeletal Abnormalities, Vascular Diseases are some of the diseases may be linked to Goat anti-SCARF1/SREC Antibody.