anti-Rtn4 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-RTN4 antibody; RTN4; Nogo-B;
The Rtn4 rtn4 (Catalog #MBS624324) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Nogo-B (Foocen, KIAA0886, My043, Nbla00271, Nbla10545, Neurite Outgrowth Inhibitor, Neuroendocrine-specific Protein, NI220/250, NSP, NSP-CL, Reticulon-4, Reticulon-5, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C, RTN-x, RTN-X, SP1507, SP1507) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Nogo-B can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1ug/ml
ELISA: 0.5-1ug/ml. Researchers should empirically determine the suitability of the Rtn4 rtn4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Rtn4 rtn4 product has the following accession number(s) (GI #21898579) (NCBI Accession #AAM77069.1) (Uniprot Accession #Q99P72). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Nogo-B (Foocen, KIAA0886, My043, Nbla00271, Nbla10545, Neurite Outgrowth Inhibitor, Neuroendocrine-specific Protein, NI220/250, NSP, NSP-CL, Reticulon-4, Reticulon-5, RTN4-A, RTN4-B1, RTN4-B2, RTN4-C, RTN-x, RTN-X, SP1507, SP1507) with the following immunoassay(s):
SDS-Page (Detection of Nogo-B: Cell lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml sheep anti-h/mNogo-B.)
Nogo-B (A No-Go for neurite outgrowth Isoform B; also reticulon-4 isoform 2 and RTN-xS) is a 49-51kD member of the reticulon protein family. It is widely expressed, being reported everywhere but liver. Nogo-B appears to be principally expressed in the ER, but does have a receptor (NgBR) on cell surfaces. Intracellularly, Nogo-B is known to interact with Bcl-xl and Bcl-2, and may play in role in both apoptosis and angiogenesis. Human Nogo-B is a two transmembrane, 373aa protein. It contains an N-terminal cytoplasmic domain (aa1-199), two transmembrane segments (aa200-219 and 315-335), a luminal region (aa220-314) and a C-terminal cytoplasmic domain (aa336-373). Nogo-B contains multiple phosphorylation sites and undergoes acetylation. Caspase-7 cleaves Nogo-B between Asp14Ser15, generating a 45-47kD fragment. Nogo-B is generated from a full-length 205kD Nogo-A by the deletion ofaa186-1004. Overaa1-200, human Nogo-B shares 87% aa identity with mouse Nogo-B1.
Immunogen: CHO cell-derived, recombinant human Nogo-B (rhNogo-B; aa1-200; Accession #NP_722550). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Rtn4 are readily searchable from our website. Different antibodies against the same target such as Rtn4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.