anti-RT1.B antibody product blog
Tags: Antibody; Monoclonal Antibody; RT1.B; anti-RT1.B antibody;
The RT1.B n/a (Catalog #MBS520415) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Rat RT1.B PE (Clone OX-6) (mouse IgG1) reacts with Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s RT1.B can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the RT1.B n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Rat RT1.B PE (Clone OX-6) (mouse IgG1) with the following immunoassay(s):
Testing Data
anti-rat RT1.B monoclonal antibody recognizes Ia from all rat strains tested. It labels a monomorphic determinant of the rat Ia antigen present on the majority of peripheral B lymphocytes, dendritic cells, some macrophages, and certain epithelial cells (2,3,4) but not peripheral T lymphocytes. (4) This antibody also cross reacts with murine Ia antigens (particularly with k and s haplotypes). Analysis of recombinant strains shows that the determinant maps to the I-A region of the mouse MHC (1,2) and correlates with mouse Ia specificity 17 or 18. Thus, the determinant recognized by MBS520415 is polymorphic in the mouse. This antibody may be useful for recognizing the rat Ia antigen and can be coupled to Sepharose 4B for purification of rat Ia antigen by antibody affinity chromatography (1).
Immunogen: rat thymocyte membrane glycoprotein
Donor: BALB/c spleen
Fusion Partner: P3-NS1-1-AG4 (NS1/1)
Strains Tested: Fischer, Wistar, Buffalo Positive: Fischer, Wistar, Buffalo Negative: None. Presentation: Biotin/FITC/PE conjugated IgG buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RT1.B are readily searchable from our website. Different antibodies against the same target such as RT1.B may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.