|The RSPO1 rspo1 (Catalog #MBS603109) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The RSPO-3 (R-Spondin 3, Cristin-1) reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s RSPO-3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).
Suitable for use in Direct ELISA and Western Blot.
Dilution: Western Blot: 1ug/ml
Optimal dilutions to be determined by researcher. Researchers should empirically determine the suitability of the RSPO1 rspo1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RSPO1 rspo1 product has the following accession number(s) (GI #339276103) (NCBI Accession #NP_001229839.1) (Uniprot Accession #Q2MKA7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
RSPO-3 (R-spondin 3; also Cristin-1), is a 256aa secreted R-Spondin family member which binds LRP-6 and Fzd8 and regulates b-catenin signaling. R-Spondins contain two adjacent cysteine-rich furin-like domains at their amino-termini, followed by a thrombospondin (TSP-1) motif and a variable length basic C-terminal region. Two RSPO-3 splice isoforms that differ in their C-termini have been reported. Mouse RSPO-3 shares 93% and 97% aa identity with human and rat RSPO-3, respectively, over the sequence used as an immunogen.
Immunogen: Recombinant corresponding to aa21-209 of mouse RSPO-3 expressed in E. coli (Accession #BAC36296). Hybridoma: Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with purified, E. coli-derived, recombinant mouse RSPO-3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RSPO1 are readily searchable from our website. Different antibodies against the same target such as RSPO1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Brain, Connective Tissue, Heart, Intestine, Muscle, Ovary, Pancreas, Parathyroid, Prostate, Testis tissues are correlated with this protein. Adenocarcinoma, Hyperplasia, Hypertrophy, Liver Diseases, Musculoskeletal Abnormalities, Necrosis, Neoplasms, Nervous System Diseases, Ovarian Diseases, Urogenital Abnormalities are some of the diseases may be linked to RSPO-3 (R-Spondin 3, Cristin-1). RSPO1 also interacts with the following gene(s): DKK1, FOXL2, FURIN, LRP6, MYF5, SOX9, SRY, STRA8, ZNRF3.