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anti-RNF34 antibody product blog

Posted on 2014-07-14 09:05:05 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; RNF34; anti-RNF34 antibody;
The RNF34 rnf34 (Catalog #MBS240834) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Human RNF34 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s RNF34 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA).
ELISA (1:4000 - 1:20000), IHC-P (10 ug/ml), WB (1:500 - 1:3000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib.. Researchers should empirically determine the suitability of the RNF34 rnf34 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The RNF34 rnf34 product has the following accession number(s) (GI #37595537) (NCBI Accession #NP_079402.2) (Uniprot Accession #Q969K3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Polyclonal to Human RNF34 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-RNF34 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunohistochemistry (IHC) RNF34.

Western Blot (WB) (Anti-RNF34 Antibody - Western Blot. Western blot of Protein A Purified anti-RNF34 antibody shows detection of human RNF34 (arrowhead) in lysate. Lanes corresponding to empty vector 293T cell lysate (mock, left); RNF34 transfected lysate (middle) and RFFL transfected lysate (right), are shown using 20 ul of lysate per lane. Lysates were prepared from equivalent numbers of cells. Data presented demonstrate that this reagent is specific for RNF34. After SDS-PAGE and transfer, the membrane was probed with the primary antibody diluted to 1:1000 using 5% BLOTTO, 0.1% Tween-20 in PBS as the diluent. Incubation occurred for 1 h at room temperature. Personal Communication, Srinivasa Srinivasula, CCR-NCI, Bethesda, MD.)
Western Blot (WB) RNF34.

Target Species: Human
Immunogen Description: Recombinant human RNF34. Immunogen Type: Recombinant protein
Immunogen: RNF34 antibody was raised against recombinant human RNF34.
Antigen Modification: aa1-373. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RNF34 are readily searchable from our website. Different antibodies against the same target such as RNF34 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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