anti-RNF216 antibody product blog
Tags: Antibody; Polyclonal Antibody; TRIAD3A; anti-RNF216 antibody; RNF216;
The RNF216 rnf216 (Catalog #MBS151271) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TRIAD3A Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s TRIAD3A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).TRIAD3A antibody can be used for detection of TRIAD3A by Western blot at 0.5 to 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 10 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the RNF216 rnf216 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RNF216 rnf216 product has the following accession number(s) (GI #31324099) (NCBI Accession #AAP47174) (Uniprot Accession #Q9NWF9). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the TRIAD3A Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of TRIAD3A in mouse heart tissue lysate with TRIAD3A antibody at (A) 1 and (B) 2 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of TRIAD3A in mouse heart with TRIAD3A antibody at 10 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of TRIAD3A in Mouse Heart cells with TRIAD3A antibody at 20 μg/mL.)
TRIAD3A Antibody: Activation of NF-κB as a result of Toll-like receptor (TLR) and IL-1 receptor signaling is a major component of innate immune responses. Signals from these receptors are relayed by a number of adapter molecules such as TRIF, TIRAP, and MyD88. Several regulatory mechanisms exist to control TLR signal transduction, including the inhibition of TLR expression and signaling by molecules such as ST2 and SIGIRR. Another mechanism is by the ubi-quitinization of selected TLRs by TRIAD3A, an E3 ubiquitin-protein ligase. TRIAD3A is a RING finger protein that can bind to TLR4 and TLR9, and to a lesser extent TLR3 and TLR5, catalyzing the ubiquitization of these molecules. Overexpression of TRIAD3A promoted the nearly complete degradation of TLR4 and TLR9; this reduction was reflected in the decreased signal-specific activation by ligands specific for these TLRs. Conversely, depletion of TRIAD3A resulted in enhanced TLR activation.
Conjugate: Unconjugated
Immunogen: TRIAD3A antibody was raised against a peptide corresponding to 15 amino acids near the amino-terminus of mouse TRIAD3A.
Buffer: TRIAD3A Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RNF216 are readily searchable from our website. Different antibodies against the same target such as RNF216 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.