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anti-RNASEH2A antibody product blog

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Posted on 2014-10-19 06:20:33 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; RNAse H2A; anti-RNASEH2A antibody; RNASEH2A;
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The RNASEH2A rnaseh2a (Catalog #MBS151275) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RNAse H2A Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s RNAse H2A can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).
RNAse H2A antibody can be used for detection of RNAse H2A by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 2 mug/mL. For immunofluorescence start at 4 mug/mL. Researchers should empirically determine the suitability of the RNASEH2A rnaseh2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The RNASEH2A rnaseh2a product has the following accession number(s) (GI #20981704) (NCBI Accession #O75792.2) (Uniprot Accession #O75792). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RNAse H2A Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of RNAse H2A in HeLa cell lysate with RNAse H2A antibody at 1 μg/mL.)
Western Blot (WB) RNASEH2A.

Immunocytochemistry (ICC) (Immunocytochemistry of RNAse H2A in HeLa cells with RNAse H2A antibody at 2 μg/mL.)
Immunocytochemistry (ICC) RNASEH2A.

Immunofluorescence (IF) (Immunofluorescence of Rnse H2A in Hela cells with Rnse H2A antibody at 4.75 μg/mL.)
Immunofluorescence (IF) RNASEH2A.

RNAse H2A Antibody: Ribonucleases (RNAses) H are enzymes that hydrolyze the RNA strands of RNA/DNA hybrids. The major role of these enzymes is to remove the RNA strand from the RNA/DNA hybrids that form during DNA replication and repair. RNAse H2 is made up of three subunits; all three are required for RNAse activity. Recent evidence has demonstrated that mutations in RNAse H2A or any of the other subunits result in Aicardi-Goutieres syndrome (AGS), a neurological disorder with similar symptoms to viral brain infections including high levels of IFN-alpha in the cerebral spinal fluid. Similar conditions are observed with mutations in TREX1, a single-stranded DNA exonuclease, suggesting that RNAse H2 and TREX1 may have similar roles, and that mutations in any of these genes lead to an accumulation of intracellular nucleic acids, triggering an inflammatory response through activation of the innate immune system.

Conjugate: Unconjugated
Immunogen: RNAse H2A antibody was raised against a 17 amino acid synthetic peptide near the center of human RNAse H2A.
Buffer: RNAse H2A Antibody is supplied in PBS containing 0.02% sodium azide.
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