anti-Rho Kinase/ROCKII (IN) antibody product blog
Tags: Antibody; Polyclonal Antibody; Rho Kinase/ROCKII (IN); anti-Rho Kinase/ROCKII (IN) antibody;
The Rho Kinase/ROCKII (IN) n/a (Catalog #MBS462238) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti ROCKII Polyclonal antibody reacts with Human, Mouse, Rat, Chicken, Bovine, Canine and may cross-react with other species as described in the data sheet. MyBioSource\'s Rho Kinase/ROCKII (IN) can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry.Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 0.5-2 ug/ml. Researchers should empirically determine the suitability of the Rho Kinase/ROCKII (IN) n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti ROCKII Polyclonal antibody with the following immunoassay(s):
Testing Data
Rho A-dependent kinase (ROCKII), or ROK-alpha, is identified a serine/threonine kinase, a downstream effector of the small GTPase Rho, mediating effects of Rho on the actin cytoskeleton, formation of actin stress fibers and focal adhesions, ROCKII alters cardiac myofilament response to Ca2+ by mechanisms involving phosphorylation of thin filament proteins. ROCK-II induces a depression in maximum ATPase rate and tension, which is associated with phosphorylation of troponin T (TnT), troponin I (TnI), and myosin-binding protein C (C-protein). The phosphorylation of Rho-kinase at Thr 249, Thr 396 plays important role in G-protein signaling..
Antigen Preparation: A synthetic peptide (19mer) derived from 250-350 amino acids of human RockII protein. This sequence is identical among human, mouse, rat, chicken, dog and bovine. Positive Control: 3T3 cell. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Rho Kinase/ROCKII (IN) are readily searchable from our website. Different antibodies against the same target such as Rho Kinase/ROCKII (IN) may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.