anti-RBC antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-RBC antibody; RBC; RBC, IgG Fraction;
The RBC n/a (Catalog #MBS524034) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Human RBC, IgG Fraction, (Polyclonal) (rabbit IgG) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s RBC, IgG Fraction can be used in a range of immunoassay formats including, but not limited to, Hemagglutination Assay (HA), Cytotoxic Assay.Hemagglutination: | |
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Test Cell Suspension: | 100 ul of washed 1-2% fresh human red blood cells (HRBC) in saline. |
Product Tested: | Diluted antibody (lot#: 0212-G) in 100 ul of heat inactivated normal rabbit serum diluted 1:100 in saline. |
Incubation: | 37�C for 20 mins, in U-bottom microtiter plates. 20�C for 2 to 4 hrs. for cell settling pattern. |
Titer: | The highest dilution of the product that yields agglutination. |
Results: | Titer 1:6400 (2%HRBC); 1:12800(1%HRBC). |
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
MBS524034 is rabbit IgG directed against human red blood cells. This IgG fraction was isolated from a high titer antiserum by salt fractionation and DEAE ion exchange chromatography. It was titered against its immunogens, sterile filtered, and analyzed for immunoglobulin purity and protein concentration. This IgG fraction contains agglutinating and lysing antibodies and can be used for these procedures.
Preservatives: None (0.2 um filtered). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RBC are readily searchable from our website. Different antibodies against the same target such as RBC may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.