|The Ras85D n/a (Catalog #MBS605436) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ras, pan, N,H,K reacts with Drosophila, Human, Monkey, Mouse, Rat, Yeast and may cross-react with other species as described in the data sheet. MyBioSource\'s Ras, pan, N,H,K can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the Ras85D n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Ras85D n/a product has the following accession number(s) (GI #17136430) (NCBI Accession #NP_476699.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The 21kD guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms. Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein.
Immunogen: Synthetic peptide corresponding to residues near the amino terminus of human K-Ras (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Ras85D are readily searchable from our website. Different antibodies against the same target such as Ras85D may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.