|The RAD52 rad52 (Catalog #MBS221533) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s RAD52 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).
Western Blot: This item detects a band of approximately 63kDa in HeLa nuclear extrants.
ELISA: Minimum Dilution: 1/5000; Maximum Dilution: 1/20000
Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/2000. Researchers should empirically determine the suitability of the RAD52 rad52 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RAD52 rad52 product has the following accession number(s) (GI #661903001) (NCBI Accession #NP_001284348.1) (Uniprot Accession #P43351). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
MBS221533 detects Rad52, a protein which functions in DNA repair through the homologous recombination of DNA double strand breaks (DSBs). DSBs can be generated through exposure to ionizing radiation or by endogenous cellular reactions, and Rad52 acts during the latter stages of recombination after the DSBs are processed into single stranded DNA (ssDNA). Rad52 functions together with Rad51 and RPA (Replication Protein A), where it has two distinct activities. First, Rad52 and RPA form a co-complex on ssDNA, where Rad52 functions to recruit Rad51. In addition, Rad52 has ssDNA binding and annealing activities, particularly ssDNA saturated with RPA. Rad52 is present at low levels during the G1 phase of the cell cycle, when recombinatorial repair is absent. The level rises steadily through S phase reaching a maximum in G2 phase when homologous repair is also at its highest level.
Perservative Stabilisers: 0.01% Sodium Azide (NaN3). Immunogen: Synthetic peptide corresponding to amino acids 360-375 of the human Rad52 protein.
Buffer Solution: Phosphate buffered saline
Antiserum Preparation: Antisera to human Rad52 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RAD52 are readily searchable from our website. Different antibodies against the same target such as RAD52 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. RAD52 also interacts with the following gene(s): ABL1, DMC1, MLH1, RAD50, RAD51, RPA1, RPA2, SUMO1, WRN.