anti-RAD50 antibody product blog
Tags: Antibody; Polyclonal Antibody; Rad50; anti-RAD50 antibody;
The RAD50 rad50 (Catalog #MBS621095) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rad50 (Mre11, Nbs1 Complex) reacts with Human, Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s Rad50 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: Western blot 1:1000. Researchers should empirically determine the suitability of the RAD50 rad50 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The RAD50 rad50 product has the following accession number(s) (GI #1518806) (Uniprot Accession #Q92878). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rad50 (Mre11, Nbs1 Complex) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts from Jurkat and K562 cells, using MBS621095.)
The DNA repair protein Rad50 is a member of the structural maintenance of chromosomes family (SMC) and plays an important role in cell cycle checkpoint signaling and double-strand break repair in response to DNA damage (1-4). Rad50 forms a complex with Mre11 and Nbs1 that becomes activated in response to DNA damage (3). In normal human cells, the MRN complex acts to tether linear DNA molecules, providing a flexible link between DNA ends (1). Genomic instability and cancer have been shown to develop in cells with genetic mutations affecting the proteins in the MRN complex (2).
Immunogen: Synthetic peptide (KLH-coupled) corresponding to the amino terminus of human Rad50. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing RAD50 are readily searchable from our website. Different antibodies against the same target such as RAD50 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Breast Neoplasms, Chromosome Breakage, Colorectal Neoplasms, Intestinal Diseases, Leukemia, Neoplasms, Nervous System Diseases, Neurologic Manifestations, Skin Diseases, Vascular Diseases are some of the diseases may be linked to Rad50 (Mre11, Nbs1 Complex). The following patways have been known to be associated with this gene. Blood, Bone, Bone Marrow, Brain, Kidney, Liver, Lung, Prostate, Skin, Testis tissues are correlated with this protein.