anti-PYCARD antibody product blog
Tags: Antibody; Polyclonal Antibody; ASC; anti-PYCARD antibody; PYCARD;
The PYCARD pycard (Catalog #MBS150936) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ASC Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s ASC can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC).ASC antibody can be used for detection of ASC/TMS1 by Western blot at 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. Researchers should empirically determine the suitability of the PYCARD pycard for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PYCARD pycard product has the following accession number(s) (GI #10801602) (NCBI Accession #BAA87339) (Uniprot Accession #Q9ULZ3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the ASC Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of ASC in HL60 whole cell lysate in the absence (A) or presence (B) of blocking peptide with ASC antibody at 1 μg /ml.)
Immunocytochemistry (IHC) (Immunocytochemistry of ASC in HL60 cells with ASC antibody at 5 μg/mL.)
Immunohistochemistry (IHC) (Immunohistochemistry of ASC in human spleen tissue with ASC antibody at 2.5 μg/ml.)
Immunofluorescence (IF) (Immunofluorescence of ASC in human spleen tissue with ASC antibody at 20 μg/ml.)
ASC Antibody: Apoptosis is regulated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. CARD containing cell death regulators include RAIDD, RICK, BCL10, Apaf-1, ARC, caspase-9, and caspase-2. A novel CARD domain containing protein was recently identified in human and mouse and designated ASC and TMS1. Ectopic expression of ASC/TMS1 induced apoptosis through activation of caspase-9 and inhibited the survival of human breast cancer cells (3, 4). Overexpression of ASC/TMS1 induced DNA fragmentation. ASC/TMS1 is expressed in a variety of human and mouse tissues.
Conjugate: Unconjugated
Immunogen: ASC antibody was raised against a 14 amino acid peptide near the carboxy terminus of human ASC.
Buffer: ASC Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PYCARD are readily searchable from our website. Different antibodies against the same target such as PYCARD may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.