anti-PUM2 antibody product blog
Tags: Antibody; Polyclonal Antibody; PUM2; anti-PUM2 antibody;
The PUM2 pum2 (Catalog #MBS421055) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PUM2 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Cow and may cross-react with other species as described in the data sheet. MyBioSource\'s PUM2 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB).Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: In transfected HEK293 transiently expressing full-length Human PUM2 (myc and DYKDDDDK tagged), a band of approx.140kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration 0.3-0.5ug/ml. Researchers should empirically determine the suitability of the PUM2 pum2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PUM2 pum2 product has the following accession number(s) (GI #13491168) (NCBI Accession #NP_056132.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
DQNRDLKQGD DDDSK.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-PUM2 Antibody with the following immunoassay(s):
Western Blot (WB) (HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human PUM2 with DYKDDDDK tag probed (0.5ug/ml) in Lane An and probed with anti- DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.)
Immunogen: Peptide with sequence C-DQNRDLKQGDDDDSK, from the internal region of the protein sequence according to NP_056132.1.
Epitope: Internal region. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PUM2 are readily searchable from our website. Different antibodies against the same target such as PUM2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. PUM2 also interacts with the following gene(s): CPEB1, CPEB3, DAZ1, DAZL, NANOS1, NANOS2, NANOS3. Blood, Brain, Embryonic Tissue, Eye, Kidney, Lung, Muscle, Ovary, Testis tissues are correlated with this protein. Adenocarcinoma, Breast Neoplasms, Carcinoma, Cell Transformation, Neoplastic are some of the diseases may be linked to Goat anti-PUM2 Antibody.