|The PSME3 psme3 (Catalog #MBS616737) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PA28, gamma, Mid (Proteasome Activator 28-gamma subunit) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PA28, gamma, Mid can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP).
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1-3ug/ml
Immunoprecipitation: 7ug/reaction. Researchers should empirically determine the suitability of the PSME3 psme3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PSME3 psme3 product has the following accession number(s) (GI #14124986) (Uniprot Accession #P61289). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Proteasome activator 28 (PA28), also known as 11S regulator, is composed of two homologous subunits, alpha (a) and beta (b), that share ~ 50% amino acid identity.1 A separate but related protein, PA28gamma, was originally identified as Ki antigen from sera of patients with systemic lupus erythematosus.1-3 PA28a and PA28b are located primarily in the cytoplasm, whereas PA28g is located in the nucleus.4,5 PA28 binds to the outer rings on both ends of the 20S proteasome to form a football-like structure.6,7 Binding of PA28 greatly stimulates multiple peptidase activities of the 20S proteasome in an ATP-independent reaction, but lacks the ability to degrade large protein substrates, suggesting that PA28 may cooperate with the 26 S proteasome in a sequential proteolytic pathway.8,9 In addition to its nuclear localization, PA28g differs from PA28a and PA28b in that it is not responsive to stimulation with IFN-g.10 Experiments with mice lacking the PA28g gene (Psme3) demonstrate that PA28g may be involved in cell cycle control; although PA28g is not essential for development, its absence causes retardation of cell proliferation and body growth.10 Abnormally high expression of PA28g has been observed in thyroid cancer, particularly in regions of cancer cells where growth rate is accelerated.11 PA28g interacts directly with Hepatitis C virus (HCV) core protein, which becomes retained in the cell\'s nucleus,12 and with MEKK3, which phosphorylates PA28g and increases its cellular levels.13.
Immunogen: Synthetic peptide derived from an internal region of the human, mouse, rat and pig PA28g (proteosome activator 28g, Ki antigen) protein. Positive Control: Human MeWo malignant melanoma, A375 malignant melanoma, Jurkat T-lymphocyte leukemia, HeLa cervical carcinoma, and HL-60 acute myeloid leukemia cell lysates. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PSME3 are readily searchable from our website. Different antibodies against the same target such as PSME3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.