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anti-PRMT5 antibody product blog

Posted on 2013-08-06 00:19:13 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; PRMT5; anti-PRMT5 antibody;
The PRMT5 prmt5 (Catalog #MBS421999) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-PRMT5 Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Pig, Dog, Cow and may cross-react with other species as described in the data sheet. MyBioSource\'s PRMT5 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB).
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 70kDa band observed in Jurkat lysates (calculated MW of 72.7kDa and 71.3kDa according to NP_006100.2; NP_001034708.1). Recommended concentration: 1-3ug/ml. Researchers should empirically determine the suitability of the PRMT5 prmt5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The PRMT5 prmt5 product has the following accession number(s) (GI #20070220) (NCBI Accession #NP_006100.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-PRMT5 Antibody with the following immunoassay(s):
Western Blot (WB) (Staining (2ug/ml) of Jurkat lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)
Western Blot (WB) PRMT5.

Immunogen: Peptide with sequence RACREKDRDPEAQ, from the internal region of the protein sequence according to NP_006100.2; NP_001034708.1.
Epitope: Internal region. Note: This antibody is expected to recognize both isoforms (NP_006100.2; NP_001034708.1). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PRMT5 are readily searchable from our website. Different antibodies against the same target such as PRMT5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Blood, Brain, Embryonic Tissue, Heart, Kidney, Liver, Lung, Muscle, Prostate, Testis tissues are correlated with this protein. PRMT5 also interacts with the following gene(s): CLNS1A, MAGOH, MBD2, PRDM1, SMARCA4, SNRPB, SNRPD1, SNRPD3, TP53, WDR77. Atrophy, Breast Neoplasms, Carcinoma, Hepatocellular, Cell Transformation, Neoplastic, Hypertension, Inflammation, Liver Diseases, Mental Disorders, Necrosis, Nervous System Diseases are some of the diseases may be linked to Goat anti-PRMT5 Antibody.
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