anti-PRLR antibody product blog
Tags: Antibody; Polyclonal Antibody; prlr; Prolactin Receptor; anti-PRLR antibody;
The PRLR prlr (Catalog #MBS176232) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Prolactin Receptor antibody reacts with Reacts with: Human, MousePredicted to work with: Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Prolactin Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin.
Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Hu
Predicted Species: Ms, Rat
Immunohistochemistry (Paraffin-embedded Section):
Concentration: 0.5-1ug/ml
Tested Species: Hu, Rat
Predicted Species: Ms
Antigen Retrieval: By Heat
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users. Researchers should empirically determine the suitability of the PRLR prlr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PRLR prlr product has the following accession number(s) (GI #730343) (NCBI Accession #Q08501.1) (Uniprot Accession #Q08501). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Prolactin Receptor antibody with the following immunoassay(s):
Western Blot (WB) (Figure 1. Western blot analysis of PRLR using anti- PRLR antibody (MBS176232).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat PC-12 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- PRLR antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRLR at approximately 90KD. The expected band size for PRLR is at 70KD.)
Immunohistochemistry (IHC) (Anti-PRLR antibody, MBS176232, IHC(P)
IHC(P): Human Mammary Cancer Tissue )
Immunohistochemistry (IHC) (Anti-PRLR antibody, MBS176232, IHC(P)
IHC(P): Rat Testis Tissue )
Description: Rabbit IgG polyclonal antibody for Prolactin receptor(PRLR) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: PRLR(Prolactin Receptor), is a cytokine receptor. By somatic cell hybrid analysis and by in situ hybridization, Arden et al.(1989, 1990) demonstrated that the prolactin receptor gene resides in the same chromosomal region as the growth hormone receptor gene, which has been mapped to 5p13-p12. Cunningham et al.(1990) demonstrated that zinc greatly increases the affinity of GH for the extracellular binding domain of PRLR, although it is not required for binding of GH to the growth hormone receptor or for binding of prolactin to the prolactin receptor. By mutational analysis, they showed that a cluster of 3 residues(histidine-18, histidine-21, and glutamic acid-174) in GH and histidine-188 in PRLR(conserved in all PRL receptors but not GH receptors) are likely zinc-ion ligands.