anti-Prekallikrein antibody product blog
Tags: Antibody; Prekallikrein; Polyclonal Antibody; anti-Prekallikrein antibody;
The Prekallikrein n/a (Catalog #MBS512124) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase.Suitable as a source of enriched antibodies to human prekallikrein. Researchers should empirically determine the suitability of the Prekallikrein n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Prekallikrein (PK), previously known as Fletcher Factor, is a single chain glycoprotein produced in the liver. The plasma concentration of PK is 50 ug/ml (550 nM), approximately 75% of which circulates in complex with high molecular weight kininogen (HK) and the remainder as free PK. Plasma PK is heterogeneous in both mass and charge due to variable degrees of glycosylation. Approximately 90% of plasma PK has an apparent molecular weight of 88 kDa as determined by SDS-PAGE and the remaining 10% has an apparent mass of 85 kDa. The catalytic site resides in the light chain. The heavy chain of PK contains four appledomain structures similar to those found in FXI and these are required for binding of PK to HK. PK is the zymogen form of the enzyme kallikrein, which is involved in the proteolysis of kininogens with subsequent release of bradykinin, a potent vasodilator. PK participates in the contact phase of coagulation as a substrate for surface-bound activated factor XII (FXIIa) in the presence of the cofactor HK. As PK and factor XI (FXI) both circulate in complex with HK, both are localized to activating surfaces through their respective binding to HK. Limited proteolysis of PK by FXIIa generates kallikrein, a two-chain serine protease that initiates the reciprocal activation of PK and FXI. Kallikrein activity in plasma is regulated predominantly by C1- Inhibitor and alpha2macroglobulin, with relatively minor contributions by Protein C Inhibitor, alpha2antiplasmin, and antithrombin1-3.
Immunogen: Human active site-blocked kallikrein prepared from plasma. Buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol.
Neutralizing Activity: Not determined. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Prekallikrein are readily searchable from our website. Different antibodies against the same target such as Prekallikrein may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.