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anti-PPP2CA antibody product blog

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Posted on 2013-12-07 01:20:08 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; PP2A; anti-PPP2CA antibody; PPP2CA;
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The PPP2CA ppp2ca (Catalog #MBS175641) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PP2A antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s PP2A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the PPP2CA ppp2ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The PPP2CA ppp2ca product has the following accession number(s) (GI #54038809) (NCBI Accession #P67775.1) (Uniprot Accession #P67775). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-PP2A antibody with the following immunoassay(s):
Western Blot (WB) (Anti-PP2A-alpha antibody, MBS175641, Western blotting
All lanes: Anti PP2A-alpha(MBS175641) at 0.5ug/ml
Lane 1: Rat kidney Tissue Lysate at 50ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 36KD
Observed bind size: 36KD)
Western Blot (WB) PPP2CA.

Immunohistochemistry (IHC) (Anti-PP2A-alpha antibody, MBS175641, IHC(P)
IHC(P): Human Mammary Cancer Tissue )
Immunohistochemistry (IHC) PPP2CA.

Immunohistochemistry (IHC) (Anti-PP2A-alpha antibody, MBS175641, IHC(P)
IHC(P): Rat Kidney Tissue )
Immunohistochemistry (IHC) PPP2CA.

Description: Rabbit IgG polyclonal antibody for Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform(PPP2CA) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: The catalytic subunit of human protein phosphatase 2A(PPP2CA) encodes a 309-amino acid polypeptide.It is localized to chromosome 5. The gene(approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue(Leu309) of protein serine/threonine phosphatase 2A catalytic subunit(PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human PP2A (6-20aa FTKELDQWIEQLNEC), identical to the related rat and mouse sequences. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PPP2CA are readily searchable from our website. Different antibodies against the same target such as PPP2CA may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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