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anti-PPM1B antibody product blog

Posted on 2015-05-21 21:52:15 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; PPM1B; anti-PPM1B antibody;
The PPM1B ppm1b (Catalog #MBS200092) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PPM1B antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PPM1B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF).
WB: 1:1,000 ~ 3,000.
Recommended starting dilution is 1:2,000. Researchers should empirically determine the suitability of the PPM1B ppm1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The PPM1B ppm1b product has the following accession number(s) (GI #29558099) (NCBI Accession #NP_808908) (Uniprot Accession #O75688). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PPM1B antibody with the following immunoassay(s):
Western Blot (WB) (Cell lysates of MCF7 (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1B (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PPM1B antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: A431 cell lysate)
Western Blot (WB) PPM1B.

Immunofluorescence (IF) (ICC/IF analysis of PPM1B in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPM1B antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF) PPM1B.

Protein phosphatase 1B (PPM1B) is a member of the PP2C family of serine/threonine protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death.

Antigen Species: Human
Immunogen: Recombinant human PPM1B (1-192aa) purified from E. coli. Clone Note: Anti-human PPM1B mAb, clone k1B1, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPM1B protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PPM1B are readily searchable from our website. Different antibodies against the same target such as PPM1B may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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