anti-PNPO antibody product blog
Tags: Antibody; Monoclonal Antibody; PNPO; anti-PNPO antibody;
The PNPO pnpo (Catalog #MBS200200) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The PNPO antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PNPO can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB).WB: 1:500 ~ 1:5000.
Recommended starting dilution is 1:1000. Researchers should empirically determine the suitability of the PNPO pnpo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PNPO pnpo product has the following accession number(s) (GI #8922498) (NCBI Accession #NP_060599) (Uniprot Accession #Q9NVS9). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PNPO antibody with the following immunoassay(s):
Western Blot (WB) (The extracts of HepG2 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human PNPO (1:500 ~ 1:5000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
PNPO (pyridoxamine 5\'-phosphate oxidase) is a 261 amino acid protein belonging to the pyridoxamine 5\'-phosphate oxidase family. It is the rate-limiting enzyme in vitamin B6 synthesis. Vitamin B6 (Pyridoxal 5-prime-phosphate or PLP) is vital for normal cellular function, and some cancer cells have notable differences in vitamin B6 metabolism compared to their normal counterparts. Vitamin B6 is a required co-factor for enzymes involved in both homocysteine metabolism and synthesis of neurotransmitters such as catecholamine.
Antigen Species: Human
Immunogen: Recombinant human PNPO (57-261aa) purified from E. coli. Clone Note: Anti-human PNPO mAb, clone AT2C7, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PNPO protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PNPO are readily searchable from our website. Different antibodies against the same target such as PNPO may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Abnormalities, Multiple, Carcinoma, Hepatocellular, Fibrosis, Hypertension, Infertility, Female, Infertility, Male, Liver Diseases, Liver Neoplasms, Pyridoxamine 5-Prime-Phosphate Oxidase Deficiency, Status Epilepticus are some of the diseases may be linked to PNPO antibody. Brain, Eye, Heart, Intestine, Kidney, Liver, Lung, Muscle, Ovary tissues are correlated with this protein.