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anti-PNPLA2 antibody product blog

Posted on 2014-10-25 04:03:16 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; anti-PNPLA2 antibody; PNPLA2; ATGL/Desnutrin;
The PNPLA2 pnpla2 (Catalog #MBS420222) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-ATGL/Desnutrin Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. MyBioSource\'s ATGL/Desnutrin can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC).
Peptide ELISA: Antibody detection limit dilution 1:32000.
Immunohistochemistry: In paraffin embedded Human Heart shows intense staining of distinct spots in the myocardial fibres in transverse section. Recommended concentration, 0.5-2ug/ml.
Western Blot: Approx 60kDa band observed in Human Adipose lystates (calculated MW of 55.3kDa according to NP_065109.1). Recommended concentration: 0.5-2ug/ml. Researchers should empirically determine the suitability of the PNPLA2 pnpla2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The PNPLA2 pnpla2 product has the following accession number(s) (GI #32698724) (NCBI Accession #NP_065109.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-ATGL/Desnutrin Antibody with the following immunoassay(s):
Western Blot (WB) ((0.5ug/ml) staining of Human Adipose lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB) PNPLA2.

Immunohistochemistry (IHC) ((2.5ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC) PNPLA2.

Immunogen: Peptide with sequence C-NIIEVSKEARKR, from the internal region of the protein sequence according to NP_065109.1. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PNPLA2 are readily searchable from our website. Different antibodies against the same target such as PNPLA2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Adipose Tissue, Blood, Brain, Connective Tissue, Eye, Heart, Kidney, Liver, Muscle, Skin tissues are correlated with this protein. Atherosclerosis, Fatty Liver, Heart Diseases, Hypertrophy, Inflammation, Insulin Resistance, Liver Diseases, Necrosis, Neutral Lipid Storage Disease with Myopathy, Non-alcoholic Fatty Liver Disease are some of the diseases may be linked to Goat anti-ATGL/Desnutrin Antibody.
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