anti-PLK1 antibody product blog
Tags: Antibody; Polyclonal Antibody; PLK1; anti-PLK1 antibody; Polo-Like Kinase Kinase (Ser482,486,490);
The PLK1 plk1 (Catalog #MBS502103) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospho-Ser482,486,490 Polo-Like Kinase Kinase reacts with Xenopus and may cross-react with other species as described in the data sheet. MyBioSource\'s Polo-Like Kinase Kinase (Ser482,486,490) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Quality Control: Western blots performed on each lot.
WB: 1:1000. Researchers should empirically determine the suitability of the PLK1 plk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PLK1 plk1 product has the following accession number(s) (GI #148235751) (NCBI Accession #NP_001080788.1) (Uniprot Accession #P70032). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Affinity purified rabbit polyclonal antibody.
Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser482,486,490 of Xenopus polo-like kinase kinase (PLKK).
Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser482/486/490 conjugated to KLH
Immunogen Species: Xenopus. Reactivity Assumed Based on 100% Sequence Homology: Rat, mouse human zebra fish
Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with Xenopus tissue. It is anticipated that the antibody will react with human, mouse, rat and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance: Considerable evidence indicates that a polo-like kinase (PLK) plays an important role in cell cycle regulation. PLK is also required for bipolar spindle formation, activation of the anaphase-promoting complex/cyclosome, and cytokinesis. Recent work led to the identification of a PLKK that is thought to be responsible for activation of PLK. Recent work (Erikson, et al., 2004) has shown that PLKK is in turn activated by phosphorylation at three sites (Ser482, Ser486 and Ser490). Thus activation of PLK is thought to involve a kinase cascade involving the phosphorylation of Ser482,486,490 in PLKK. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PLK1 are readily searchable from our website. Different antibodies against the same target such as PLK1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Breast Neoplasms, Carcinoma, Carcinoma, Hepatocellular, Cell Transformation, Neoplastic, Disease Models, Animal, Liver Neoplasms, Lung Neoplasms, Neoplasms, Neoplasms, Experimental, Prostatic Neoplasms are some of the diseases may be linked to Anti-Phospho-Ser482,486,490 Polo-Like Kinase Kinase.