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anti-pH2AX antibody product blog

Posted on 2014-01-21 13:11:14 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; pH2AX; anti-pH2AX antibody;
The pH2AX n/a (Catalog #MBS837487) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The pH2AX antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s pH2AX can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
WB: 1:5000. Researchers should empirically determine the suitability of the pH2AX n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the pH2AX antibody with the following immunoassay(s):
Western Blot (WB) ()
Western Blot (WB) pH2AX.

Rabbit polyclonal pH2AX antibody.

Biological Significance: H2AX becomes phosphorylated on serine 139, then called gamma-H2AX, as a reaction on DNA Double-strand breaks (DSB). The kinases of the PI3-family (Ataxia telangiectasia mutated, ATR and DNA-PKcs) are responsible for this phosphorylation, especially ATM. The modification can happen accidentally during replication fork collapse or in the response to ionizing radiation but also during controlled physiological processes such as V(D)J recombination. Immunogen: pH2AX antibody was raised in Rabbit using a synthetic target peptide as the immunogen. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing pH2AX are readily searchable from our website. Different antibodies against the same target such as pH2AX may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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