anti-PGR antibody product blog
Tags: Antibody; PGR; Monoclonal Antibody; anti-PGR antibody; Progesterone Receptor (Ser190);
The PGR pgr (Catalog #MBS500011) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospho-Ser190 Progesterone Receptor reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Progesterone Receptor (Ser190) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC (frozen sections; unpublished observations): 1:1000. Researchers should empirically determine the suitability of the PGR pgr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PGR pgr product has the following accession number(s) (GI #110611914) (NCBI Accession #NP_000917.3) (Uniprot Accession #P06401). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Phospho-Ser190 Progesterone Receptor with the following immunoassay(s):
Testing Data
Affinity purified mouse monoclonal antibody.
Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser190 of human progesterone receptor.
Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser190 conjugated to KLH
Immunogen Species: Human. Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with human tissue.
Biological Significance: There is accumulating evidence to suggest that progesterone plays an essential role in the regulation of growth and differentiation of mammary glands and thus may play a key role in breast cancer (Edwards, 2005). The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (PR-An and PR-B forms). In most cell contexts, the B form functions as a transcriptional activator, whereas the A form functions as a transcriptional inhibitor of steroid hormones (Attia et al., 2000; Lin et al., 2003). Recently it has been demonstrated that there is differential hormone dependent regulation of the phosphorylation of the An and B forms of the receptor (Clemm et al., 2000). Treatment of T47D breast cancer cells with progestin agonist increases the phosphorylation of Ser190 and Ser294 with different kinetics. These phosphorylation events may differentially affect the transcriptional activity of the receptor. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PGR are readily searchable from our website. Different antibodies against the same target such as PGR may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.