anti-PF4 antibody product blog
Tags: Antibody; Platelet Factor 4; PF4; Polyclonal Antibody; anti-PF4 antibody;
The PF4 pf4 (Catalog #MBS512119) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase.Suitable as a source of antibodies to human Platelet Factor 4. Researchers should empirically determine the suitability of the PF4 pf4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PF4 pf4 product has the following accession number(s) (GI #4505733) (NCBI Accession #NP_002610.1) (Uniprot Accession #P02776). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Platelet Factor 4 (PF4) is a small high-affinity heparin binding protein localized in the alpha-granules of platelets and megacaryocytes. It is a member of a multigene "intercrinecytokine" family that includes beta-thromboglobulin, connective tissue activating peptide III, monocyte-derived neutrophil chemotactic factor and melanoma growth stimulatory factor. Human PF4 consists of 70 amino acids and has a mass of 7800 daltons. In the platelet, PF4 exists as a homo-tetramer in complex with a high-molecular weight proteoglycan carrier. PF4 is one of the most abundant proteins in the platelet alpha-granule and is considered platelet-specific, as its concentration in platelets is more than 20,000-fold greater than in plasma. The heparin neutralization activity of PF4 is believed to be due to the presence of four lysine residues interspersed among pairs of aliphatic residues in the C-terminal region of the molecule. During vessel injury, platelets are stimulated by thrombin or other agonists to release alpha-granule contents at the site of injury. Presumably the potent heparin neutralization activity of PF4 would reduce the anticoagulant activity of antithrombin, prolonging the action of thrombin and activated factor X to promote clot formation. Released PF4 also binds to heparin-like molecules on the surface of endothelial cells and hepatocytes, neutralizing the anticoagulant activity expressed by these cells. Some of the other activities attributable to PF4 include the potentiation of platelet aggregation, stimulation of fibroblast attachment to substrata, chemotactic activity with respect to neutrophils and inhibition of contact activation1,2.
Immunogen: Platelet Factor 4 purified from human platelet releasate. Buffer: 10 mM HEPES, pH 7.4, 150 mM NaCl, 50% (v/v) glycerol
Neutralizing Activity: Not determined. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PF4 are readily searchable from our website. Different antibodies against the same target such as PF4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.