anti-PEBP1 antibody product blog
Tags: Antibody; PEBP1; Polyclonal Antibody; anti-PEBP1 antibody;
The PEBP1 pebp1 (Catalog #MBS125273) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PEBP1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s PEBP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), RIP.WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
IP: 1:20 - 1:50
RIP: 1:20 - 1:50. Researchers should empirically determine the suitability of the PEBP1 pebp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PEBP1 pebp1 product has the following accession number(s) (GI #1352726) (NCBI Accession #P30086.3) (Uniprot Accession #P30086). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PEBP1 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using PEBP1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.)
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells using PEBP1 antibody. Blue: DAPI for nuclear staining.)
Immunoprecipitation (IP) (Immunoprecipitation analysis of 150ug extracts of MCF-7 cells using 3ug PEBP1 antibody. Western blot was performed from the immunoprecipitate using PEBP1 antibody at a dilition of 1:500.)
Raf kinase inhibitor protein (RKIP) is a member of the phosphatidylethanolamine-binding protein (PEBP) family that associates with Raf-1 and the MEK and MAP kinases (1). RKIP has been shown to complex with Raf-1, MEK, and ERK (2). Although MEK and ERK can simultaneously bind RKIP, the association between Raf-1 and RKIP and that of RKIP and MEK are mutually exclusive. Thus, RKIP competitively disrupts the Raf-1-MEK complex and effectively terminates signal transmission from Raf-1 to MAP kinases (2). The inhibitory effect of RKIP on MAP kinase signaling is eliminated by PKC phosphorylation of RKIP at Ser153 (3). PKC phosphorylation on Ser153 also promotes the association of RKIP with GRK2, which prevents GRK2-dependent internalization of GPCR (4). RKIP also interacts with modules of the NF-kappaB pathway, including NF-kappaB-inducing kinase (NIK), TAK1, IKKalpha and IKKbeta (5). These interactions antagonize cytokine-induced activation of the NF-kappaB pathway (5). Restoration of RKIP expression is associated with the inhibition of prostate cancer metastasis, implying that RKIP may be a potential clinical target as a suppressor of tumor metastasis through inhibition of vascular invasion (6).
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human PEBP1
Calculated Molecular Weight: 21kDa.