|The PDPN pdpn (Catalog #MBS245474) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 5E2] (IgG2b,k) to Human PDPN / Podoplanin reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s PDPN / Podoplanin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA).
ELISA, IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the PDPN pdpn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PDPN pdpn product has the following accession number(s) (GI #54792056) (NCBI Accession #NP_006465.3) (Uniprot Accession #Q86YL7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Monoclonal [clone 5E2] (IgG2b,k) to Human PDPN / Podoplanin with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-Podoplanin antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Western Blot (WB) (The extracts of mouse kidney (50 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PDPN (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Target Species: Human
Immunogen Description: Recombinant human PDPN (1-206aa) purified from E Coli. Immunogen Type: Recombinant protein
Immunogen: PDPN / Podoplanin antibody was raised against recombinant human PDPN (1-206aa) purified from E Coli.
Antigen Modification: aa1-206. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PDPN are readily searchable from our website. Different antibodies against the same target such as PDPN may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Adenocarcinoma, Carcinoma, Disease Models, Animal, Immune System Diseases, Inflammation, Lung Diseases, Lung Neoplasms, Neoplasms, Experimental, Ovarian Diseases, Precancerous Conditions are some of the diseases may be linked to Mouse Monoclonal [clone 5E2] (IgG2b,k) to Human PDPN / Podoplanin.