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anti-PDE4B antibody product blog

Posted on 2013-11-06 22:29:19 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; Phosphodiesterase 4B; anti-PDE4B antibody; PDE4B;
The PDE4B pde4b (Catalog #MBS420285) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-Phosphodiesterase 4B Antibody reacts with Tested: Mouse; Expected from sequence similarity: Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Phosphodiesterase 4B can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB).
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 85kDa band observed in Mouse Brain lysates (calculated MW of 83.3kDa according to Human NP_002591.2 and 82.1kDa according to Mouse NP_062814.2). In transfected HEK293 transiently expressing Human PDE4B a band of approx.85kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.5-1.0 ug/ml. Researchers should empirically determine the suitability of the PDE4B pde4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The PDE4B pde4b product has the following accession number(s) (GI #32171241) (NCBI Accession #NP_002591.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Goat anti-Phosphodiesterase 4B Antibody with the following immunoassay(s):
Western Blot (WB) ((0.5ug/ml) staining of Mouse Brain Lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB) PDE4B.

Western Blot (WB) (HEK293 overexpressing Human PDE4B (RC211956) and probed (mock transfection in first lane), tested by Origene.)
Western Blot (WB) PDE4B.

Immunogen: Peptide with sequence C-DIDIATEDKSPVDT, from the C Terminus of the protein sequence according to NP_002591.2; NP_001032418.1; NP_001032416.1; NP_001032417.1.
Epitope: C Terminus. Note: This antibody is expected to recognise all reported isoforms (NP_002591.2, NP_001032418.1, NP_001032416.1 and NP_001032417.1). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PDE4B are readily searchable from our website. Different antibodies against the same target such as PDE4B may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. PDE4B also interacts with the following gene(s): ADCY2, ADCY7, ADCY9. Blood, Bone, Brain, Heart, Kidney, Liver, Lung, Muscle, Testis, Vascular tissues are correlated with this protein.
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