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anti-PARP4 antibody product blog

Posted on 2013-11-29 18:29:42 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; PARP4; anti-PARP4 antibody;
The PARP4 n/a (Catalog #MBS120501) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse monoclonal antibody Anti-Human PARP4 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PARP4 can be used in a range of immunoassay formats including, but not limited to, Dot Blot (DB). Researchers should empirically determine the suitability of the PARP4 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse monoclonal antibody Anti-Human PARP4 with the following immunoassay(s):
Quality Control (Western blot analysis of immunized recombinant protein, using anti-PARP4 monoclonal antibody.)
Quality Control PARP4.

Quality Control #2 (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)
Quality Control #2 PARP4.

Immunocytochemistry (ICC) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-PARP4 mAb. [LotNo. 2695C5a-1])
Immunocytochemistry (ICC) PARP4.

This gene encodes poly(ADP-ribosyl)transferase-like 1 protein, which is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. Since this protein is not capable of binding DNA directly, its transferase activity may be activated by other factors such as protein-protein interaction mediated by the extensive carboxyl terminus. [provided by RefSeq][NCBI Entrez Gene Summary].

Preparation: This antibody was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Sterility: Filtered through a 0.22 um membrane. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PARP4 are readily searchable from our website. Different antibodies against the same target such as PARP4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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