anti-PAK2 antibody product blog
Tags: Antibody; Polyclonal Antibody; PAK2; anti-PAK2 antibody;
The PAK2 pak2 (Catalog #MBS221479) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI PAK2 (C-TERMINAL) reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s PAK2 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Paraffin*, Western Blot (WB).Western Blot: This item detects a band of approximately 58kDa in Jurkat cell lysates.
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml. Researchers should empirically determine the suitability of the PAK2 pak2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PAK2 pak2 product has the following accession number(s) (GI #32483399) (NCBI Accession #NP_002568.2) (Uniprot Accession #Q13177). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RABBIT ANTI PAK2 (C-TERMINAL) with the following immunoassay(s):
Testing Data #1 (western blot analysis of whole cell lysate from Jurkat human T-cell leukemia cells probed with Rabbit anti Human PAK2 (MBS221479) at 0.5(A) and 1(B) ug/ml)
Testing Data #2 (Formalin fixed, paraffin embedded mouse spleen stained with Rabbit anti Human PAK2 (MBS221479) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
MBS221479 recognises p21-activated kinase-2 (PAK2), an ubiquitously expressed, 64kDa, serine/threonine protease found at higher levels in skeletal muscle, ovary, thymus and spleen. p21-activated kinases (PAKs) bind activated Cdc42 and Rac via a Cdc42/Rac interactive binding (CRIB) domain. Group 1 PAK kinases (PAK1, 2 and 3) regulate the activity of an adjacent conserved autoinhibitory domain and are associated with cellular processes, including cytoskeleton rearrangement, apoptosis and MAP kinase signalling. Group 2 PAKs (PAK4, 5, and 6) lack the autoinhibitory domain and are not activated by Cdc42/Rac binding. PAK2 has a cytostatic effect on cells and is activated in response to cellular stresses such hyperosmolarity, serum starvation, contact inhibition and DNA damage from radiation and chemicals. Unlike other PAKs, PAK2 can be activated by proteolytic cleavage to generate a constitutively active fragment, PAK2p34, which induces a cell death response. This induction is thought to be regulated by Rho GTPase-activating protein 10 which inhibits the protein kinase activity of PAK2p34 and causes it to localise to the perinuclear region instead of the nucleus. PAK2 may also play a role in X-linked mental retardation.