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anti-PAFAH1B1 antibody product blog

Posted on 2013-09-16 16:08:12 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; anti-PAFAH1B1 antibody; PAFAH1B1; PAFAH1B1 / LIS1;
The PAFAH1B1 pafah1b1 (Catalog #MBS241299) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal (IgG) to Human PAFAH1B1 / LIS1 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s PAFAH1B1 / LIS1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB).
IHC-P (5 ug/ml), WB (1 ug/ml)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the PAFAH1B1 pafah1b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The PAFAH1B1 pafah1b1 product has the following accession number(s) (GI #4557741) (NCBI Accession #NP_000421.1) (Uniprot Accession #P43034). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Polyclonal (IgG) to Human PAFAH1B1 / LIS1 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-PAFAH1B1 / LIS1 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC) PAFAH1B1.

Immunofluorescence (IF) (Immunofluorescence of LIS1 in Jurkat cells with LIS1 antibody at 20 ug/ml.)
Immunofluorescence (IF) PAFAH1B1.

Immunocytochemistry (ICC) (Immunocytochemistry of LIS1 in Jurkat cells with LIS1 antibody at 2.5 ug/ml.)
Immunocytochemistry (ICC) PAFAH1B1.

Western Blot (WB) (Western blot of LIS1 in HeLa cell lysate with LIS1 antibody at (A) 0.5 and (B) 1 ug/ml.)
Western Blot (WB) PAFAH1B1.

PAFAH1B1 was identified as encoding a gene that when mutated or lost caused the lissencephaly associated with Miller-Dieker lissencephaly syndrome. PAFAH1B1 encodes the non-catalytic alpha subunit of the intracellular Ib isoform of platelet-activating factor acteylhydrolase, a heterotrimeric enzyme that specifically catalyzes the removal of the acetyl group at the SN-2 position of platelet-activating factor (identified as 1-O-alkyl-2-acetyl-sn-glyceryl-3-phosphorylcholine).

Target Species: Human
Immunogen Description: Synthetic peptide from human PAFAH1B1 / LIS1. Immunogen Type: Synthetic peptide
Immunogen: PAFAH1B1 / LIS1 antibody was raised against synthetic peptide from human PAFAH1B1 / LIS1.
Antigen Modification: C-Terminus. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PAFAH1B1 are readily searchable from our website. Different antibodies against the same target such as PAFAH1B1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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