anti-PAFAH1B1 antibody product blog
Tags: Antibody; Polyclonal Antibody; LIS1; anti-PAFAH1B1 antibody; PAFAH1B1;
The PAFAH1B1 pafah1b1 (Catalog #MBS150442) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LIS1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s LIS1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).LIS1 antibody can be used for detection of LIS1 by Western blot at 0.5 - 1 mug/mL. Antibody can also be used for immunocytochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the PAFAH1B1 pafah1b1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PAFAH1B1 pafah1b1 product has the following accession number(s) (GI #1170794) (NCBI Accession #P43034.2) (Uniprot Accession #P43034). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the LIS1 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of LIS1 in HeLa cell lysate with LIS1 antibody at (A) 0.5 and (B) 1 μg/mL.)
Immunocytochemistry (IHC) (Immunocytochemistry of LIS1 in Jurkat cells with LIS1 antibody at 2.5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of LIS1 in Jurkat cells with LIS1 antibody at 20 μg/mL.)
LIS1 Antibody: Lissencephaly is a severe brain developmental disease characterized by the mislocalization of cortical neurons, a smooth cerebral surface, mental retardation, and seizures. Classical lissencephaly is caused by sporadic mutations in the LIS1 gene. While LIS1 is known to act in a pathway deactivating the lipid messenger platelet-activating factor, LIS1 forms a complex with Nudel and 14-3-3epsilon which is then transported from neuronal cell bodies through the actions of DISC1 and KIF5A, a microtubule-dependent directed motor protein kinesin. Decreased expression of LIS1 blocked neural stem cell division, morphogenesis, and motility, suggesting that LIS1 plays an important role in neuronal cell proliferation and localization in the developing brain. At least two isoforms of LIS1 are known to exist.
Conjugate: Unconjugated
Immunogen: LIS1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human LIS1.
Buffer: LIS1 Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PAFAH1B1 are readily searchable from our website. Different antibodies against the same target such as PAFAH1B1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.